Journal: Npj Gut and Liver
Article Title: Silencing of S100A11 attenuates murine metabolic dysfunction-associated steatohepatitis
doi: 10.1038/s44355-025-00044-w
Figure Lengend Snippet: A Representative BODIPY stained images from Hu1545 human hepatocyte cell line treated with palmitate (PA) with or without benitrobenrazide (BNBZ). The experiment was replicated 3 times. Vehicle control (Veh) included equivalent amounts of isopropanol and DMSO. Scale bar: 20 µm. B Quantification of BODIPY staining was performed in Image J. Each dot represents a separate experiment averaged over five fields. Approximately 16–36 cells were counted per field. Data were calculated as the mean intensity divided by the number of cells per field. ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: S100A11 knockout cells were generated in the human Hu1545 cell line using Guide-it® CRISPR/Cas9 System (Takara Bio) with these single guide sequences 5’—AATTAGACAGAGTTCCTAAGCTTC and 3’—AATTGAAGCTTAGGAACTCTGTCT.
Techniques: Staining, Control